Vol. 2, 2017

Original research papers

Biochemistry

RELATIONSHIP BETWEEN BEHAVIORS AND CATECHOLAMINE CONTENT IN PREFRONTAL CORTEX AND HIPPOCAMPUS OF CHRONICALLY STRESSED RATS

Nataša Popović, Snežana B. Pajović, Vesna Stojiljković, Ana Todorović, Snežana Pejić, Ivan Pavlović, Ljubica Gavrilović

Pages: 255-259

DOI: 10.21175/RadProc.2017.52

Chronic stress induces over-activation and dysfunction of stress-activated systems, resulting in further brain damage and depressive-like behavior. Depression is a potentially life-threatening disorder that affects people and, therefore, it is one of the most important public health problems. This study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on the anxiety-like and depression-like behaviors in rats, as well as on the possible changes in the concentrations of dopamine (DA) and noradrenaline (NA) in the prefrontal cortex and hippocampus. We observed a decrease in the number of entries into open arms and time spent in open arms during the elevated plus-maze test (anxiety-like behavior), as well as the increased immobility during the forced swimming test (depression-like behavior). In addition, we found that CRS increases concentration of NA and decreases concentration of DA in the prefrontal cortex and hippocampus. Also, we recorded a significant correlation between the animal behavior and levels of neurotransmitters in the prefrontal cortex and hippocampus in stress conditions provoked by CRS. The results presented here suggest that there is a relationship between the animal behavior and levels of neurotransmitters in the prefrontal cortex and hippocampus in stress conditions provoked by CRS, which may be important in the research of numerous psychiatric diseases caused by chronic stress.
  1. D. Ongür, J. L. Price, “The organization of networks within the orbital and medial prefrontal cortex of rats, monkeys and humans,” Cereb. Cortex, vol. 10, no. 3, pp. 206-219, Mar. 2000.
    DOI: 10.1093/cercor/10.3.206
    PMid: 10731217
  2. H. Eichenbaum, “Hippocampus: cognitive processes and neural representations that underlie declarative memory,” Neuron, vol.44, no. 1,pp.109-120, Sep. 2004.
    DOI: 10.1016/j.neuron.2004.08.028
    PMid: 15450164
  3. Y. C. Tse, I. Montoya, A. S. Wong, A. Mathieu, J. Lissemore, D. C. Lagace, T. P. Wong, “A longitudinal study of stress-induced hippocampal volume changes in mice that are susceptible or resilient to chronic social defeat,” Hippocampus, vol.24, no.9, pp.1120-1128, Sep. 2014.
    DOI: 10.1002/hipo.22296
    PMid: 24753271
  4. R. Jankord, J. P. Herman, “Limbic regulation of hypothalamo–pituitary–adrenocortical function during acute and chronic stress,” Ann. N. Y. Acad. Sci., vol. 1148, pp. 64–73, Dec. 2008.
    DOI: 10.1196/annals.1410.012
    PMid: 19120092
    PMCid: PMC2637449
  5. M. R. Levinstein, B. A. Samuels, “Mechanisms underlying the antidepressant response and treatment resistance,” Front. Behav. Neurosci. vol. 8, p.208, Jun. 2014.
    DOI: 10.3389/fnbeh.2014.00208
    PMid: 25018708
    PMCid: PMC4073308
  6. Y. Liu, X. Zhuang, L. Gou, X. Ling, X. Tian, L. Liu, Y. Zheng, L. Zhang, X. Yin, “Protective effects of nizofenone administration on the cognitive impairments induced by chronic restraint stress in mice,” Pharmacol. Biochem. Behav. vol. 103, no. 3, pp. 474–480, Jan. 2013.
    DOI: 10.1016/j.pbb.2012.09.009
    PMid: 23026061
  7. A. C. Ferraz, A. M. Delattre, R. G. Almendra, M. Sonagli, C. Borges, P. Araujo, M. L. Andersen, S. Tufik, M. M. Lima, “Chronic omega-3 fatty acids supplementation promotes beneficial effects on anxiety, cognitive and depressive-like behaviors in rats subjected to a restraint stress protocol,” Behav. Brain Res., vol. 219, no. 1, pp. 116–122, May 2011.
    DOI: 10.1016/j.bbr.2010.12.028
    PMid: 21192985
  8. Y. Wang, H. Kan, Y. Yin, W. Wu, W. Hu, M. Wang, W. Li, W. Li, “Protective effects of ginsenoside Rg1 on chronic restraint stress induced learning and memory impairments in male mice,” Pharmacol. Biochem. Behav., vol. 120, pp. 73–81, May, 2014.
    DOI: 10.1016/j.pbb.2014.02.012
    PMid: 24560910
  9. G. D. Gamaro, M. B. Michalowski, D. H. Catelli, M. H. Xavier, C. Dalmaz, “Effect of repeated restraint stress on memory in different tasks,” Braz. J. Med. Biol. Res., vol. 32, no. 3, pp. 341-347, Mar. 1999.
    DOI: 10.1590/S0100-879X1999000300015
    PMid: 10347794
  10. K. S. Kim, P. L. Han, “Optimization of chronic stress paradigms using anxiety- and depression-like behavioral parameters,” J. Neurosci. Res., vol. 83, no. 3, pp. 497-507, Feb. 2006.
    DOI: 10.1002/jnr.20754
    PMid: 16416425
  11. S. Pellow, P. Chopin, S. E. File, M. Briley, “Validation of open:closed arm entries in an elevated plus-maze as a measure of anxiety in the rat,” J. Neurosci. Methods., vol. 14, no.3,pp.149-167, Aug. 1985.
    DOI: 10.1016/0165-0270(85)90031-7
  12. H. Cohen, A. B. Geva, M. A. Matar, J. Zohar, Z. Kaplan, “Post-traumatic stress behavioural responses in inbred mouse strains: can genetic predisposition explain phenotypic vulnerability?” Int. J. Neuropsychopharmacol., vol.11, no. 3, pp.331-349, May 2008.
    DOI: 10.1017/S1461145707007912
    PMid: 17655807
  13. H. Cohen, M. A. Matar, D. Buskila, Z. Kaplan, J. Zohar, “Early post-stressor intervention with high-dose corticosterone attenuates posttraumatic stress response in an animal model of posttraumatic stress disorder,” Biol. Psychiatry., vol.64, no. 8, pp. 708-717, Oct. 2008.
    DOI: 10.1016/j.biopsych.2008.05.025
    PMid: 18635156
  14. A. Mazor, M. A. Matar, Z. Kaplan, N. Kozlovsky, J. Zohar, H. Cohen, “Gender-related qualitative differences in baseline and post-stress anxiety responses are not reflected in the incidence of criterion-based PTSD-like behaviour patterns,” World J. Biol. Psychiatry., vol.10, no. 4-3, pp.856-869, Dec. 2009.
    DOI: 10.1080/15622970701561383
    PMid: 17886167
  15. J. F. Cryan, A. Markou, I. Lucki, “Assessing antidepressant activity in rodents: recent developments and future needs,” Trends Pharmacol. Sci., vol. 23, no. 5, pp. 238-245, May 2002.
    DOI: 10.1016/S0165-6147(02)02017-5
  16. R. D. Porsolt, G. Anton, N. Blavet, M. Jalfre, “Behavioural despair in rats: a new model sensitive to antidepressant treatments,” Eur. J. Pharmacol., vol. 47, no. 4, pp.379-391, Feb. 1978.
    DOI: 10.1016/0014-2999(78)90118-8
  17. M. J. Detke, M. Rickels, I. Lucki, “Active behaviors in the rat forced swimming test differentially produced by serotonergic and noradrenergic antidepressants,” Psychopharmacology, vol.121, no. 1, pp.66-72, Sep. 1995.
    DOI: 10.1007/BF02245592
  18. G. Piras, O. Giorgi, M. G. Corda, “Effects of antidepressants on the performance in the forced swim test of two psychogenetically selected lines of rats that differ in coping strategies to aversive conditions,” Psychopharmacology, vol. 211, no. 4, pp.403-414, Sep. 2010.
    DOI: 10.1007/s00213-010-1904-x
    PMid: 20589496
  19. S. Chiba, T. Numakawa, M. Ninomiya, M. C. Richards, C. Wakabayashi, H. Kunugi, “Chronic restraint stress causes anxiety- and depression-like behaviors, downregulates glucocorticoid receptor expression, and attenuates glutamate release induced by brain-derived neurotrophic factor in the prefrontal cortex,” Progress in Neuro-Psychopharmacology and Biological Psychiatry, vol. 39, no. 1, pp. 112-119, Oct. 2012.
    DOI: 10.1016/j.pnpbp.2012.05.018
    PMid: 22664354
  20. B. Haenisch, A. Bilkei-Gorzo, M. G. Caron, H. Bönisch, “Knockout of the norepinephrine transporter and pharmacologically diverse antidepressants prevent behavioral and brain neurotrophin alterations in two chronic stress models of depression,” J. Neurochem., vol. 111, no. 2, pp. 403-416, Oct. 2009.
    DOI: 10.1111/j.1471-4159.2009.06345.x
    PMid: 19694905
    PMCid: PMC2764285
  21. G. Patki, F. Atrooz, I. Alkadhi, N. Solanki, S. Salim, “High aggression in rats is associated with elevated stress, anxiety-like behavior, and altered catecholamine content in the brain,” Neurosci. Lett., vol. 584, no. 1, pp. 308-313, Jan. 2015.
    DOI: 10.1016/j.neulet.2014.10.051
    PMid: 25450144
    PMCid: PMC4322760
  22. G. Tanda, E. Carboni, R. Frau, G. Di Chiara, “Increase of extracellular dopamine in the prefrontal cortex: a trait of drugs with antidepressant potential?” Psychopharmacology, vol. 115, no. 1, pp. 285–288, Jun. 1994.
    DOI: 10.1007/BF02244785
  23. K. W. Lange, T. W. Robbins, C. D. Marsden, M. James, A. M. Owen, G. Paul, “L-dopa withdrawal in Parkinson`s disease selectively impairs cognitive performance in tests sensitive to frontal lobe dysfunction,” Psychopharmacology, vol. 107, no. 2, pp. 394–404, Jun. 1992.
    DOI: 10.1007/BF02245167
  24. M. Rusnák, R. Kvetnanský, J. Jeloková, M. Palkovits, “Effect of novel stressors on gene expression of tyrosine hydroxylase and monoamine transporters in brainstem noradrenergic neurons of long-term repeatedly immobilized rats,” Brain Res., vol. 899, no. 1-2, pp 20-35, Apr. 2001.
    DOI: 10.1016/S0006-8993(01)02126-6
  25. B. E. Leonard, “Stress, norepinephrine and depression,” J. Psychiatry Neurosci., vol. 26, pp.11-16, 2001.
    PMCid: PMC2553257
  26. H. Eichenbaum, “Hippocampus: cognitive processes and neural representations that underlie declarative memory,” Neuron, vol. 44, no. 1, pp.109-120, Sep. 2004.
    DOI: 10.1016/j.neuron.2004.08.028
    PMid: 15450164
  27. Y. C. Tse, I. Montoya, A. S. Wong, A. Mathieu, J. Lissemore, D. C. Lagace, T. P. Wong, “A longitudinal study of stress-induced hippocampal volume changes in mice that are susceptible or resilient to chronic social defeat,” Hippocampus, vol. 24, no. 9, pp.1120-1128, Sep. 2014.
    DOI: 10.1002/hipo.22296
    PMid: 24753271