Vol. 2, 2017

Original research papers



L. G. Stoyanova, L. P. Blinkova, Yu. D. Pakhomov, S. Dbar

Pages: 19-24

DOI: 10.21175/RadProc.2017.05

Bacteriocin-producing strains of Lactococcus lactis subsp. lactis of different origion were used in our experiments. UV-Irradiation of cells of bacteriocin-synthesis lactoococci of different origion in dose of 7.6–7600 erg/mm2 followed by their selective growth in MRS medium and selection after UV-rays treatment. Some strain of lactococci had double UV treatment. The novel most active bacteriocin-producing strains were restored after lyophilization and storage. An effective dose of treatment of wide strain 229 should be considered to an exposure of 10 minutes. As a result, No.12 with an antibiotic activity of 3850 IU / mL was selected, which is 54 % higher than the initial one. The study of the physiological and biochemical properties of variants, selected after UV-rays treatment, showed that they somewhat changed the rate of their growth and the accumulation of bactericine. The relative activity in the production of bacteriocine was calculated as the ratio of activity values to the number of produced cells. The inactivated homologous strain biomass and aminoacids were applied as the factors of resuscitation. Control - culture liquid without supplements. VBNC cells of opportunistic strains formed with the first days of incubation. After 1 year of incubation Klebsiella pneumoniae 1954, Alcaligenes faecalis 415, Enterobacter aerogenes 418, Proteus vulgaris HX19222, Salmonella typhimurium value VBNC values were statistically equal (97.1 - 99.9%). The strains of L. lactis after 1 day of stress didn’t form colonies up to 60-80%, after 5 days to 82.1 - 99.6%, after 1 year – 99,9%. Unwashed from the culture fluid the inoculated cells proliferated and passed to VBNC faster. With that inoculation the nisin productive activity of cells was lower at 10 - 78 times, depending on strain of L. lactis. The study of resuscitation factors have shown that supplement of homologous inactivated biomass of L. lactis (0.1%, 0.5%, 1%) was effectively with 1% (a magnification of 2.65 at p<0.05) and 0.5% (magnification of 3.75 at p<0.05) only for strain MSU. For strain F 116 marked 4 fold increase in the ability to the cultivation after the addition of 7 aminoacids: (glutamine, methionine, leucine, isoleucine, histidine, arginine, valine). The quantitative level of VBNC cells, which formed in the first days of incubation, after 1 year was the same for opportunistic and probiotic cultures (97.1% - 99.9%). All studied resuscitation factors were individually effective for bacterial strains.
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