Vol. 2, 2017



Mihai Surcel, Didi Surcel, Sebastian Toader, Mioara Butan

Pages: 243-248

DOI: 10.21175/RadProc.2017.50

The aim of this study was to evaluate the mechanism by which EMF therapy can interfere with the modified immune and oxidative reactions by the experimental approach on the lab animals. In vivo and vitro experiment was carried out on 60 Wistar rats that were divided in 4 groups as following: 1. Control-group tested on the 10th day; 2.ELF-EMF-exposed group tested on the 10th day; 3. Group that received intradermic Staphylococcus aureus culture (SA) and was tested on the 10th day; 4. Group that received intradermic SA and was treated with ELF- EMF and was tested after 10 days. The rats were exposed to 50 Hz, 1 mT. Splenic lymphocytes and alveolar macrophages for the cellular cultures were harvested. The following parameters were assessed: a) 3HTdR incorporation test; b) Macrophage inhibition factor (MIF) assay; c)Inteleukine-1 (IL-1)-assay; d) Tumor Necrosis Factor (TNF)- assay; e) Chemiluminescence assay; f) Phagocytosis assay. The 3HTdR incorporation assay and assays for cytokine activity and phagocytosis indicate a partial reversal of the values of the results obtained in the ELF + EMF group. Our experiment reveals that the EMF interferes with the immune and oxidative systems, becomes chronic and plays a key role in the development of serious diseases. The EMF-therapy can be beneficial in reducing the inflammation by interfering with the inflammatory molecules and surface receptors.
  1. M. Simkó, M. Mattsson, “Extremely low frequency electromagnetic fields as effectors of cellular responses in vitro: possible immune cell activation,” J. Cell Biochem., vol. 93, no. 1, pp 83 – 92, Sep. 2004.
    DOI: 10.1002/jcb.20198
    PMid: 15352165
  2. D. B. Lyle, R. D. Ayotte, A. R. Sheppard, W. R. Adey, “Suppression of T-lymphocyte cytotoxicity following exposure to 60-Hz sinusoidal electric fields,” Bioelectromagnetics, vol. 9, no. 3, pp. 303 – 313, 1988.
    DOI: 10.1002/bem.2250090311
    PMid: 3263132
  3. C. D’Angelo, E. Costantini, M. A. Kamal, “Experimental model for elf-emf exposure: concern for human health,” Saudi J. Biol. Sci., vol. 22, no. 1, pp. 75 – 84, Jan. 2015.
    DOI: 10.1016/j.sjbs.2014.07.006
    PMid: 25561888
    PMCid: PMC4281612
  4. M. L. Guzman, S. J. Neering et al., “Nuclear factor-κB is constitutively activated in primitive human acute myelogenous leukemia cells,” Blood, vol. 98, no. 8, pp. 2301 – 2307, Oct. 2001.
    DOI: 10.1182/blood.V98.8.2301
    PMid: 11588023
  5. A. Maziarz, B. Kocan, M. Bester, S. Budzik, “How electromagnetic fields can influence adult stem cells: positive and negative impacts,” Stem Cell Res. Ther., vol. 7, no. 1, p. 54, Apr. 2016.
    DOI: 10.1186/s13287-016-0312-5
    PMid: 27086866
    PMCid: PMC4834823
  6. S. Gangi, O. Johansson, “A theoretical model based upon mast cells and histamine to explain the recently proclaimed sensitivity to electric and/or magnetic fields in humans,” Medical Hypotheses, vol. 54, no. 4, pp. 663 – 671, Apr. 2000.
    DOI: 10.1054/mehy.1999.0923
    PMid: 10859662
  7. O. Johansson, “Disturbance of the immune system by electromagnetic fields—A potentially underlying cause for cellular damage and tissue repair reduction which could lead to disease and impairment,” Pathophysiology, vol. 16, no. 2-3, pp. 157 – 177, Aug. 2009.
    DOI: 10.1016/j.pathophys.2009.03.004
    PMid: 19398310
  8. C. L. Ross, B. S. Harrison, “An introduction to electromagnetic field therapy and immune function: a brief history and current status,” J. of Science and Applications: Biomedicine, vol. 3, no. 2, pp. 18 – 29, 2015.
    Retrieved from: http://inter-use.com/uploads/soft/150302/1-150302163S4.pdf
    Retrieved on: Jan. 20, 2017
  9. M. Pesce, A. Patruno, L. Speranza, M. Reale, “Extremely Low Frequency Electromagnetic Field and Wound Healing: Implication of Cytokines as Biological Mediators,” European Cytokine Network, vol. 24, no. 1, pp. 1 – 10, Mar. 2013.
    DOI: 10.1684/ecn.2013.0332
    PMid: 23674517
  10. F. Guerriero, G. Ricevuti, “Extremely low frequency electromagnetic fields stimulation modulates autoimmunity and immune responses: a possible immuno-modulatory therapeutic effect in neurodegenerative diseases,” Neural Regen. Res., vol. 11, no. 12, pp. 1888 – 1895, Dec. 2016.
    DOI: 10.4103/1673-5374.195277
    PMid: 28197174
    PMid: PMC5270416