Vol. 1, 2016

Original research papers

Radiobiology

PROTON INDUCTION OF GENE MUTATIONS

Natalia Koltovaya, Alexandra Kokoreva, Natalia Shvaneva, Nadya Zhuchkina

Pages: 5-6

DOI: 10.21175/RadProc.2016.02

We investigated the effect of low-LET proton irradiation on gene mutations. DNA lesions were caused by the acute exposure of the cells to 150 MeV proton irradiation (linear energy transfer of 0.539 keV/μm) and 60Co γ-rays at doses up to 25 Gy. To detect gene mutations, we used a forward mutation rate assay that detects the mutations inactivating the arginine permease gene (CanR mutations) and a reversion assay detecting the frameshift mutations that reverts a 4-base insertion in the LYS2 gene (lys2-Bgl). The dose responses can be described by a linear relationship for both types of gene mutations. Different radiation sources that induce mutations have the relative biological effectiveness values of 1.25 for frameshift mutations and 1.69 for forward gene mutations CanR. So, the damage induced by proton irradiation of cells appears to be more serious than the damage induced by γ-irradiation, which is indicated by an increase in the yield of not only DSBs but also gene mutations.
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